Preparation of liquid crystal droplets and its application in biological detection

YANG Xiu-xiu; TIAN Yi; YANG Zhong-qiang

doi:10.37188/CJLCD.2022-0215

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Preparation of liquid crystal droplets and its application in biological detection

Material Physics | 更新时间：2022-11-22

- Preparation of liquid crystal droplets and its application in biological detection
- Chinese Journal of Liquid Crystals and Displays Vol. 37, Issue 12, Pages: 1531-1545(2022)
- 作者机构：
  
  1.清华大学化学系有机光电子与分子工程教育部重点实验室，北京 100084
- 作者简介：
- 基金信息：
  
  National Natural Science Foundation of China(21174077);Tsinghua University Initiative Scientific Research Program
- DOI：10.37188/CJLCD.2022-0215
  CLC：
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YANG Xiu-xiu, TIAN Yi, YANG Zhong-qiang. Preparation of liquid crystal droplets and its application in biological detection. [J]. Chinese Journal of Liquid Crystals and Displays 37(12):1531-1545(2022)
DOI：

YANG Xiu-xiu, TIAN Yi, YANG Zhong-qiang. Preparation of liquid crystal droplets and its application in biological detection. [J]. Chinese Journal of Liquid Crystals and Displays 37(12):1531-1545(2022) DOI： 10.37188/CJLCD.2022-0215.

摘要

基于液晶液滴体系的生物传感器因其具有较高的检测灵敏度、特异的光学信号，在检测细菌、病毒、酶活性、蛋白质及生物分子相互作用等方面展现其独特优势。液晶液滴生物传感器性能与液晶液滴的尺寸、均一性及其界面的特异识别能力密切相关。因此，制备具有尺寸可控、界面化学可调的液晶液滴成为当前研究的重点。本文从液晶液滴的取向介绍出发，着重概述了近年来液晶液滴制备的研究进展及其在生物检测领域应用中的发展状况。同时介绍了液晶液滴复合材料的制备和液晶液滴的表面固定及其在生物传感中的应用，最后讨论了液晶液滴生物传感器目前遇到的瓶颈以及未来可能的研究方向。

Abstract

Liquid crystal droplet biosensor has made remarkable progress in the biological detection field because of its high detection sensitivity and specific optical signal. Liquid crystal droplet has shown outstanding performance in detecting bacteria， viruses， enzymatic activity， proteins， and the interaction between biomolecules. As the performance of liquid crystal droplet biosensor is closely related to the size and uniformity of liquid crystal droplets as well as the specific recognition ability of the interface， the preparation of liquid crystal droplets with controllable size and adjustable interfacial chemistry has become the focus of current research. This review summarizes the research progress of liquid crystal droplet preparation and its application in the field of biological detection in recent years. First， this review introduces the orientation of liquid crystal droplets， then， summarizes the research progress of liquid crystal droplet preparation and its application in the field of biological detection in recent years. This review also introduces the preparation of liquid crystal droplet composites， the surface immobilization of liquid crystal droplets and their applications in biosensors. Finally， the bottlenecks encountered by liquid crystal droplet biosensors and the possible research directions in the future are discussed.

关键词

液晶液晶液滴生物传感器检测

Keywords

liquid crystaldropletsbiosensordetection

references

KIM Y K， NOH J， NAYANI K， et al. Soft matter from liquid crystals ［J］. Soft Matter， 2019， 15（35）： 6913-6929. doi: 10.1039/c9sm01424ahttp://dx.doi.org/10.1039/c9sm01424a

GUPTA V K， SKAIFE J J， DUBROVSKY T B， et al. Optical amplification of ligand-receptor binding using liquid crystals ［J］. Science， 1998， 279（5359）： 2077-2080. doi: 10.1126/science.279.5359.2077http://dx.doi.org/10.1126/science.279.5359.2077

HUSSAIN A， PINA A S， ROQUE A C A. Bio-recognition and detection using liquid crystals ［J］. Biosensors and Bioelectronics， 2009， 25（1）： 1-8. doi: 10.1016/j.bios.2009.04.038http://dx.doi.org/10.1016/j.bios.2009.04.038

QU R X， GEORGE T F， LI G Q. Development in liquid crystal microcapsules： fabrication， optimization and applications ［J］. Journal of Materials Chemistry C， 2022， 10（2）： 413-432. doi: 10.1039/d1tc04395ahttp://dx.doi.org/10.1039/d1tc04395a

ZHANG X， XU Y Y， VALENZUELA C， et al. Liquid crystal-templated chiral nanomaterials： from chiral plasmonics to circularly polarized luminescence ［J］. Light： Science & Applications， 2022， 11（1）： 223. doi: 10.1038/s41377-022-00913-6http://dx.doi.org/10.1038/s41377-022-00913-6

LOCKWOOD N A， GUPTA J K， ABBOTT N L. Self-assembly of amphiphiles， polymers and proteins at interfaces between thermotropic liquid crystals and aqueous phases ［J］. Surface Science Reports， 2008， 63（6）： 255-293. doi: 10.1016/j.surfrep.2008.02.002http://dx.doi.org/10.1016/j.surfrep.2008.02.002

DONG Y C， YANG Z Q. Beyond displays： the recent progress of liquid crystals for bio/chemical detections ［J］. Chinese Science Bulletin， 2013， 58（21）： 2557-2562. doi: 10.1007/s11434-013-5767-5http://dx.doi.org/10.1007/s11434-013-5767-5

GUPTA J K， SIVAKUMAR S， CARUSO F， et al. Size-dependent ordering of liquid crystals observed in polymeric capsules with micrometer and smaller diameters ［J］. Angewandte Chemie International Edition， 2009， 48（9）： 1652-1655. doi: 10.1002/anie.200804500http://dx.doi.org/10.1002/anie.200804500

LIN I H， MILLER D S， BERTICS P J， et al. Endotoxin-induced structural transformations in liquid crystalline droplets ［J］. Science， 2011， 332（6035）： 1297-1300. doi: 10.1126/science.1195639http://dx.doi.org/10.1126/science.1195639

TJIPTO E， CADWELL K D， QUINN J F， et al. Tailoring the interfaces between nematic liquid crystal emulsions and aqueous phases via layer-by-layer assembly ［J］. Nano Letters， 2006， 6（10）： 2243-2248. doi: 10.1021/nl061604phttp://dx.doi.org/10.1021/nl061604p

DENG J N， LU X Y， CONSTANT C， et al. Design of β-CD-surfactant complex-coated liquid crystal droplets for the detection of cholic acid via competitive host-guest recognition ［J］. Chemical Communications， 2015， 51（43）： 8912-8915. doi: 10.1039/c5cc01561hhttp://dx.doi.org/10.1039/c5cc01561h

YANG X X， LIANG X， NANDI R， et al. DNA-modified liquid crystal droplets ［J］. Biosensors， 2022， 12（5）： 275. doi: 10.3390/bios12050275http://dx.doi.org/10.3390/bios12050275

UMBANHOWAR P B， PRASAD V， WEITZ D A. Monodisperse emulsion generation via drop break off in a coflowing stream ［J］. Langmuir， 2000， 16（2）： 347-351. doi: 10.1021/la990101ehttp://dx.doi.org/10.1021/la990101e

KHAN W， CHOI J H， KIM G M， et al. Microfluidic formation of pH responsive 5CB droplets decorated with PAA-b-LCP ［J］. Lab on A Chip， 2011， 11（20）： 3493-3498. doi: 10.1039/c1lc20402ehttp://dx.doi.org/10.1039/c1lc20402e

DENG J N， HAN D D， YANG J. Applications of microfluidics in liquid crystal-based biosensors ［J］. Biosensors， 2021， 11（10）： 385. doi: 10.3390/bios11100385http://dx.doi.org/10.3390/bios11100385

SIVAKUMAR S， GUPTA J K， ABBOTT N L， et al. Monodisperse emulsions through templating polyelectrolyte multilayer capsules ［J］. Chemistry of Materials， 2008， 20（6）： 2063-2065. doi: 10.1021/cm703237ahttp://dx.doi.org/10.1021/cm703237a

LI Y， MEI R A， YANG Z Q. A facile method for preparation of emulsion using the high gravity technique ［J］. Journal of Colloid and Interface Science， 2017， 506： 120-125. doi: 10.1016/j.jcis.2017.06.035http://dx.doi.org/10.1016/j.jcis.2017.06.035

WANG Z Y H， XU T H， NOEL A， et al. Applications of liquid crystals in biosensing ［J］. Soft Matter， 2021， 17（18）： 4675-4702. doi: 10.1039/d0sm02088ehttp://dx.doi.org/10.1039/d0sm02088e

SIVAKUMAR S， WARK K L， GUPTA J K， et al. Liquid crystal emulsions as the basis of biological sensors for the optical detection of bacteria and viruses ［J］. Advanced Functional Materials， 2009， 19（14）： 2260-2265. doi: 10.1002/adfm.200900399http://dx.doi.org/10.1002/adfm.200900399

ORTIZ B J， BOURSIER M E， BARRETT K L， et al. Liquid crystal emulsions that intercept and report on bacterial quorum sensing ［J］. ACS Applied Materials & Interfaces， 2020， 12（26）： 29056-29065.

BRAKE J M， ABBOTT N L. Coupling of the orientations of thermotropic liquid crystals to protein binding events at lipid-decorated interfaces ［J］. Langmuir， 2007， 23（16）： 8497-8507. doi: 10.1021/la0634286http://dx.doi.org/10.1021/la0634286

ALIÑO V J， PANG J， YANG K L. Liquid crystal droplets as a hosting and sensing platform for developing immunoassays ［J］. Langmuir， 2011， 27（19）： 11784-11789. doi: 10.1021/la2022215http://dx.doi.org/10.1021/la2022215

VERMA I， PANI I， SHARMA D， et al. Label-free imaging of fibronectin adsorption at Poly-（L-lysine）-decorated liquid crystal droplets ［J］. The Journal of Physical Chemistry C， 2019， 123（22）： 13642-13650. doi: 10.1021/acs.jpcc.9b01934http://dx.doi.org/10.1021/acs.jpcc.9b01934

COHEN J. The immunopathogenesis of sepsis ［J］. Nature， 2002， 420（6917）： 885-891. doi: 10.1038/nature01326http://dx.doi.org/10.1038/nature01326

JIANG S L， NOH J H， PARK C， et al. Using machine learning and liquid crystal droplets to identify and quantify endotoxins from different bacterial species ［J］. Analyst， 2021， 146（4）： 1224-1233. doi: 10.1039/d0an02220ahttp://dx.doi.org/10.1039/d0an02220a

张亚倩，崔永丰，王浩，等.基于卷积神经网络的高通量蓝相液晶识别［J］.液晶与显示，2022，37（8）：972-979. doi: 10.37188/CJLCD.2021-0315http://dx.doi.org/10.37188/CJLCD.2021-0315

ZHANG Y Q， CUI Y F， WANG H， et al. High-throughput blue phase liquid crystal recognition based on convolutional neural network ［J］. Chinese Journal of Liquid Crystals and Displays， 2022， 37（8）： 972-979. （in Chinese）. doi: 10.37188/CJLCD.2021-0315http://dx.doi.org/10.37188/CJLCD.2021-0315

YANG X X， ZHAO X F， ZHAO H S， et al. Combination of liquid crystal and deep learning reveals distinct signatures of Parkinson's disease-related wild-type α-synuclein and six pathogenic mutants ［J］. Chemistry-An Asian Journal， 2022， 17（2）： e202101251. doi: 10.1002/asia.202101251http://dx.doi.org/10.1002/asia.202101251

GUPTA J K， ZIMMERMAN J S， DE PABLO J J， et al. Characterization of adsorbate-induced ordering transitions of liquid crystals within monodisperse droplets ［J］. Langmuir， 2009， 25（16）： 9016-9024. doi: 10.1021/la900786bhttp://dx.doi.org/10.1021/la900786b

CHANG C Y， CHEN C H. Oligopeptide-decorated liquid crystal droplets for detecting proteases ［J］. Chemical Communications， 2014， 50（81）： 12162-12165. doi: 10.1039/c4cc04651jhttp://dx.doi.org/10.1039/c4cc04651j

YANG X X， TIAN Y， LI F Y， et al. Investigation of the assembly behavior of an amphiphilic lipopeptide at the liquid crystal-aqueous interface ［J］. Langmuir， 2019， 35（7）： 2490-2497. doi: 10.1021/acs.langmuir.8b03294http://dx.doi.org/10.1021/acs.langmuir.8b03294

MA C D， ADAMIAK L， MILLER D S， et al. Liquid crystal interfaces programmed with enzyme-responsive polymers and surfactants ［J］. Small， 2015， 11（43）： 5747-5751. doi: 10.1002/smll.201502137http://dx.doi.org/10.1002/smll.201502137

WANG Y， ZHAO L Y， XU A J， et al. Detecting enzymatic reactions in penicillinase via liquid crystal microdroplet-based pH sensor ［J］. Sensors and Actuators B： Chemical， 2018， 258： 1090-1098. doi: 10.1016/j.snb.2017.12.012http://dx.doi.org/10.1016/j.snb.2017.12.012

LEE K， SHIN H， GUPTA K C， et al. In vitro dual detection of GNPs conjugated rabbit IgG using anti-IgG anchored calcein green fluorescent LC microdroplets ［J］. IEEE Sensors Journal， 2018， 18（20）： 8208-8214.

LEE K， GUPTA K C， PARK S Y， et al. Anti-IgG-anchored liquid crystal microdroplets for label free detection of IgG ［J］. Journal of Materials Chemistry B， 2016， 4（4）： 704-715. doi: 10.1039/c5tb02131fhttp://dx.doi.org/10.1039/c5tb02131f

KHAN M， PARK S Y. Specific detection of avidin-biotin binding using liquid crystal droplets ［J］. Colloids and Surfaces B： Biointerfaces， 2015， 127： 241-246. doi: 10.1016/j.colsurfb.2015.01.047http://dx.doi.org/10.1016/j.colsurfb.2015.01.047

PANI I， FIDHA N K M， SHARMA M， et al. Probing nanoscale lipid-protein interactions at the interface of liquid crystal droplets ［J］. Nano Letters， 2021， 21（11）： 4546-4553. doi: 10.1021/acs.nanolett.0c05139http://dx.doi.org/10.1021/acs.nanolett.0c05139

MANNA U， ZAYAS-GONZALEZ Y M， CARLTON R J， et al. Liquid crystal chemical sensors that cells can wear ［J］. Angewandte Chemie International Edition， 2013， 52（52）： 14011-14015. doi: 10.1002/anie.201306630http://dx.doi.org/10.1002/anie.201306630

KHAN M， LI W W， MAO S F， et al. Real-time imaging of ammonia release from single live cells via liquid crystal droplets immobilized on the cell membrane ［J］. Advanced Science， 2019， 6（20）： 1900778. doi: 10.1002/advs.201900778http://dx.doi.org/10.1002/advs.201900778

MANNA U， ZAVALA Y M， ABBOTT N L， et al. Structured liquid droplets as chemical sensors that function inside living cells ［J］. ACS Applied Materials & Interfaces， 2021， 13（36）： 42502-42512. doi: 10.1021/acsami.1c12667http://dx.doi.org/10.1021/acsami.1c12667

VERMA I， SIDIQ S， PAL S K. Poly（L-lysine）-coated liquid crystal droplets for sensitive detection of DNA and their applications in controlled release of drug molecules ［J］. ACS Omega， 2017， 2（11）： 7936-7945. doi: 10.1021/acsomega.7b01175http://dx.doi.org/10.1021/acsomega.7b01175

YOO H， JO H， OH S S. Detection and beyond： challenges and advances in aptamer-based biosensors ［J］. Materials Advances， 2020， 1（8）： 2663-2687. doi: 10.1039/d0ma00639dhttp://dx.doi.org/10.1039/d0ma00639d

YU H X， ALKHAMIS O， CANOURA J， et al. Advances and challenges in small-molecule DNA aptamer isolation， characterization， and sensor development ［J］. Angewandte Chemie International Edition， 2021， 60（31）： 16800-16823. doi: 10.1002/anie.202008663http://dx.doi.org/10.1002/anie.202008663

KIM J， KHAN M， PARK S Y. Glucose sensor using liquid-crystal droplets made by microfluidics ［J］. ACS Applied Materials & Interfaces， 2013， 5（24）： 13135-13139. doi: 10.1021/am404174nhttp://dx.doi.org/10.1021/am404174n

WANG Y， HU Q Z， TIAN T T， et al. Simple and sensitive detection of pesticides using the liquid crystal droplet patterns platform ［J］. Sensors and Actuators B： Chemical， 2017， 238： 676-682. doi: 10.1016/j.snb.2016.07.114http://dx.doi.org/10.1016/j.snb.2016.07.114

ZHOU L L， HU Q Z， KANG Q， et al. Construction of liquid crystal droplet-based sensing platform for sensitive detection of organophosphate pesticide ［J］. Talanta， 2018， 190： 375-381. doi: 10.1016/j.talanta.2018.08.014http://dx.doi.org/10.1016/j.talanta.2018.08.014

GOLLAPELLI B， TATIPAMULA A K， DEWANJEE S， et al. Detection of bile acids using optical biosensors based on cholesteric liquid crystal droplets ［J］. Journal of Materials Chemistry C， 2021， 9（39）： 13991-14002. doi: 10.1039/d1tc02801dhttp://dx.doi.org/10.1039/d1tc02801d

NIU X F， LUO D， CHEN R， et al. Optical biosensor based on liquid crystal droplets for detection of cholic acid ［J］. Optics Communications， 2016， 381： 286-291. doi: 10.1016/j.optcom.2016.07.016http://dx.doi.org/10.1016/j.optcom.2016.07.016

DUAN R， LI Y Z， SHI B J， et al. Real-time， quantitative and sensitive detection of urea by whispering gallery mode lasing in liquid crystal microdroplet ［J］. Talanta， 2020， 209： 120513. doi: 10.1016/j.talanta.2019.120513http://dx.doi.org/10.1016/j.talanta.2019.120513

DENG J N， LIANG W L， FANG J Y. Liquid crystal droplet-embedded biopolymer hydrogel sheets for biosensor applications ［J］. ACS Applied Materials & Interfaces， 2016， 8（6）： 3928-3932. doi: 10.1021/acsami.5b11076http://dx.doi.org/10.1021/acsami.5b11076

KINSINGER M I， BUCK M E， ABBOTT N L， et al. Immobilization of polymer-decorated liquid crystal droplets on chemically tailored surfaces ［J］. Langmuir， 2010， 26（12）： 10234-10242. doi: 10.1021/la100376uhttp://dx.doi.org/10.1021/la100376u

GUO X R， MANNA U， ABBOTT N L， et al. Covalent immobilization of caged liquid crystal microdroplets on surfaces ［J］. ACS Applied Materials & Interfaces， 2015， 7（48）： 26892-26903. doi: 10.1021/acsami.5b09595http://dx.doi.org/10.1021/acsami.5b09595

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Key Laboratory of Organic Optoelectronics & Molecular Engineering of the Ministry of Education， Department of Chemistry， Tsinghua University

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