Processing and analysis of surface plasmon resonance image of polystyrene microspheres

WU Jing-ning; LIU Zi-wei; YANG Bo; CAI Chen; QI Zhi-mei

doi:10.37188/CJLCD.2022-0057

您当前的位置：

首页 >

文章列表页 >

Processing and analysis of surface plasmon resonance image of polystyrene microspheres

Image Processing | 更新时间：2022-12-07

- Processing and analysis of surface plasmon resonance image of polystyrene microspheres
- Chinese Journal of Liquid Crystals and Displays Vol. 37, Issue 9, Pages: 1174-1181(2022)
- 作者机构：
  
  1.中国科学院空天信息创新研究院传感技术国家重点实验室，北京 100190
  2.中国科学院大学电子电气与通信工程学院，北京 100049
  3.中国科学院大学光电学院，北京 100049
- 作者简介：
- 基金信息：
  
  National Natural Science Foundation of China(62121003;61931018;61871365)
- DOI：10.37188/CJLCD.2022-0057
  CLC： TH744;TP391
- Received：16 February 2022，
  
  Revised：09 March 2022，
  
  Published：05 September 2022
- 稿件说明：
移动端阅览
WU Jing-ning, LIU Zi-wei, YANG Bo, et al. Processing and analysis of surface plasmon resonance image of polystyrene microspheres[J]. Chinese journal of liquid crystals and displays, 2022, 37(9): 1174-1181.
DOI：

WU Jing-ning, LIU Zi-wei, YANG Bo, et al. Processing and analysis of surface plasmon resonance image of polystyrene microspheres[J]. Chinese journal of liquid crystals and displays, 2022, 37(9): 1174-1181. DOI： 10.37188/CJLCD.2022-0057.

摘要

使用高光谱SPRi传感器对聚苯乙烯微球进行SPR显微成像，并对不同偏振条件下采集的高光谱SPR图像数据进行了处理，有效削弱了SPR高光谱图像中固有污点的对比度，使得聚苯乙烯微球样品的位置与轮廓更加清晰。处理后的单像素SPR光谱变得平滑，降低了光源光谱本身特性以及随机噪声对SPR共振信息提取的影响。对比聚苯乙烯微球的SPR图像与反射式明场显微图像，发现二者存在明显区别，主要是由于消逝场穿透深度的限制。结果表明，相较于反射式明场显微图像，SPR图像可以明显反映出消逝场内微粒与金属表面的接触情况，但无法量化超出消逝场穿透深度的物体的真实尺寸。

Abstract

The hyperspectral SPRi sensor was used to perform microscopic analysis of polystyrene microspheres， and the hyperspectral SPR data collected under different polarization conditions were processed， resulting in effective suppression of the contrast of inherent stains in the SPR hyperspectral images and making the position and outline of the polystyrene microsphere sample clearer. The processed SPR spectra become smooth， reducing the influence of the spectral characteristics of the light source and random noise on the SPR resonance information extraction. By comparing the SPR image of polystyrene microspheres with the reflection bright field microscopic image， it is found that there are obvious differences between the two images. The main reason is the limitation of the penetration depth of the evanescent field. The results show that SPR images can clearly reflect the contact between particles and metal surfaces in the evanescent field， but cannot quantify the true size of objects beyond the penetration depth of the evanescent field compared with reflection bright field microscopy images.

关键词

Keywords

references

KRETSCHMANN E ， RAETHER H . Notizen： radiative decay of non radiative surface plasmons excited by light ［J］. Zeitschrift Für Naturforschung A ， 1968 ， 23 （ 12 ）： 2135 - 2136 . doi: 10.1515/zna-1968-1247 http://dx.doi.org/10.1515/zna-1968-1247

YEATMAN E ， ASH E A . Surface plasmon microscopy ［J］. Electronics Letters ， 1987 ， 23 （ 20 ）： 1091 - 1092 . doi: 10.1049/el:19870762 http://dx.doi.org/10.1049/el:19870762

HICKEL W ， KAMP D ， KNOLL W . Surface-plasmon microscopy ［J］. Nature ， 1989 ， 339 （ 6221 ）： 186 . doi: 10.1038/339186a0 http://dx.doi.org/10.1038/339186a0

GIEBEL K F ， BECHINGER C ， HERMINGHAUS S ， et al . Imaging of cell/substrate contacts of living cells with surface Plasmon resonance microscopy ［J］. Biophysical Journal ， 1999 ， 76 （ 1 ）： 509 - 516 . doi: 10.1016/s0006-3495(99)77219-x http://dx.doi.org/10.1016/s0006-3495(99)77219-x

LAPLATINE L ， LEROY L ， CALEMCZUK R ， et al . Spatial resolution in prism-based surface plasmon resonance microscopy ［J］. Optics Express ， 2014 ， 22 （ 19 ）： 22771 - 22785 . doi: 10.1364/oe.22.022771 http://dx.doi.org/10.1364/oe.22.022771

AHANGAR S B ， KONDURU V ， ALLEN J S ， et al . Development of automated angle-scanning， high-speed surface plasmon resonance imaging and SPRi visualization for the study of dropwise condensation ［J］. Experiments in Fluids ， 2020 ， 61 （ 1 ）： 12 . doi: 10.1007/s00348-019-2844-9 http://dx.doi.org/10.1007/s00348-019-2844-9

KANO H ， KNOLL W . Locally excited surface-plasmon-polaritons for thickness measurement of LBK films ［J］. Optics Communications ， 1998 ， 153 （ 4/6 ）： 235 - 239 . doi: 10.1016/s0030-4018(98)00240-5 http://dx.doi.org/10.1016/s0030-4018(98)00240-5

SOMEKH M G ， LIU S G ， VELINOV T S ， et al . High-resolution scanning surface-plasmon microscopy ［J］. Applied Optics ， 2000 ， 39 （ 34 ）： 6279 - 6287 . doi: 10.1364/ao.39.006279 http://dx.doi.org/10.1364/ao.39.006279

KANO H ， KNOLL W . A scanning microscope employing localized surface-plasmon-polaritons as a sensing probe ［J］. Optics Communications ， 2000 ， 182 （ 1/3 ）： 11 - 15 . doi: 10.1016/s0030-4018(00)00794-x http://dx.doi.org/10.1016/s0030-4018(00)00794-x

DE BRUIJN H E ， KOOYMAN R P ， GREVE J . Surface plasmon resonance microscopy： improvement of the resolution by rotation of the object ［J］. Applied Optics ， 1993 ， 32 （ 13 ）： 2426 - 2430 . doi: 10.1364/ao.32.002426 http://dx.doi.org/10.1364/ao.32.002426

TANAKA T ， YAMAMOTO S . Laser-scanning surface plasmon polariton resonance microscopy with multiple photodetectors ［J］. Applied Optics ， 2003 ， 42 （ 19 ）： 4002 - 4007 . doi: 10.1364/ao.42.004002 http://dx.doi.org/10.1364/ao.42.004002

SON T ， LEE C ， MOON G ， et al . Enhanced surface plasmon microscopy based on multi-channel spatial light switching for label-free neuronal imaging ［J］. Biosensors and Bioelectronics ， 2019 ， 146 ： 111738 . doi: 10.1016/j.bios.2019.111738 http://dx.doi.org/10.1016/j.bios.2019.111738

SUN X Q ， LIU H Y ， YAN Y ， et al . Imaging to single virus by using surface plasmon polariton scattering ［C］// Proceedings of SPIE 10244， International Conference on Optoelectronics and Microelectronics Technology and Application . Shanghai， China ： SPIE ， 2017 ： 1024425 . doi: 10.1117/12.2264434 http://dx.doi.org/10.1117/12.2264434

SUN X Q ， LIU H Y ， JIANG L W ， et al . Detecting a single nanoparticle by imaging the localized enhancement and interference of surface plasmon polaritons ［J］. Optics Letters ， 2019 ， 44 （ 23 ）： 5707 - 5710 . doi: 10.1364/ol.44.005707 http://dx.doi.org/10.1364/ol.44.005707

YU H ， SHAN X N ， WANG S P ， et al . Plasmonic imaging and detection of single DNA molecules ［J］. ACS Nano ， 2014 ， 8 （ 4 ）： 3427 - 3433 . doi: 10.1021/nn4062885 http://dx.doi.org/10.1021/nn4062885

ZHANG P F ， MA G Z ， DONG W ， et al . Plasmonic scattering imaging of single proteins and binding kinetics ［J］. Nature Methods ， 2020 ， 17 （ 10 ）： 1010 - 1017 . doi: 10.1038/s41592-020-0947-0 http://dx.doi.org/10.1038/s41592-020-0947-0

PETERSON A W ， HALTER M ， PLANT A L ， et al . Surface plasmon resonance microscopy： achieving a quantitative optical response ［J］. Review of Scientific Instruments ， 2016 ， 87 （ 9 ）： 093703 . doi: 10.1063/1.4962034 http://dx.doi.org/10.1063/1.4962034

ZYBIN A ， KURITSYN Y A ， GUREVICH E L ， et al . Real-time detection of single immobilized nanoparticles by surface plasmon resonance imaging ［J］. Plasmonics ， 2010 ， 5 （ 1 ）： 31 - 35 . doi: 10.1007/s11468-009-9111-5 http://dx.doi.org/10.1007/s11468-009-9111-5

SERRANO D ， SEEGER S . Supercritical angle Raman microscopy： a surface-sensitive nanoscale technique without field enhancement ［J］. Light： Science & Applications ， 2017 ， 6 （ 10 ）： e17066 . doi: 10.1038/lsa.2017.66 http://dx.doi.org/10.1038/lsa.2017.66

HOMOLA J ， YEE S S ， GAUGLITZ G . Surface plasmon resonance sensors： review ［J］. Sensors and Actuators B： Chemical ， 1999 ， 54 （ 1/2 ）： 3 - 15 . doi: 10.1016/s0925-4005(98)00321-9 http://dx.doi.org/10.1016/s0925-4005(98)00321-9

Views

441

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Development of AOI inspection of Mura defects on TFT-LCD surface

Application of dual-wavelength fiber guided light based on OpenCV in high-precision assembly and alignment

Design and implementation of piecewise approximation bilateral filtering algorithm based on FPGA

Defect detection of small object solder joints based on improved YOLOv7

Related Author

Liu Zi-wei

Yang Bo

Cai Chen

CHEN Zekang

SHEN Yi

ZHAI Chenyang

DONG Chenyao

WANG Shuangxi

Related Institution

College of Engineering， Shantou University

Guangdong Provincial Key Laboratory of Automotive Display and Touch Technologies

College of Mechanical and Electrical Engineering， Hainan University

Key Laboratory of Chemical Lasers， Dalian Institute of Chemical Physics， Chinese Academy of Sciences

School of Microelectronics， Shanghai University

AI问答

Address：No.3888 Dong Nanhu Road, Changchun, Jilin, China 130033 Postal code：130033
Tel：0431-86176059 Email：yjxs@ciomp.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 吉ICP备11002662号-17 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰