Segmentation and Description to adhesion Macrophage Image

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Segmentation and Description to adhesion Macrophage Image

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- Segmentation and Description to adhesion Macrophage Image
- Chinese Journal of Liquid Crystals and Displays Vol. 27, Issue 2, (2012)
- 作者机构：
  
  南昌大学信息工程学院电子信息工程系
- 作者简介：
- 基金信息：
- DOI：
  CLC： TP391.41
- Received：18 July 2012，
  
  Revised：12 August 2012，
  
  Published Online：27 August 2012，
  
  Published：15 April 2012
- 稿件说明：
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王英涛, 周璇, 张志东. 双轴向列相Fréedericksz转变的Monte Carlo模拟[J]. 液晶与显示, 2012,(2): 144-152

WANG Ying-tao, ZHOU Xuan, ZHANG Zhi-dong. Monte Carlo Simulation of Fréedericksz Transitions in Biaxial Nematic Liquid Crystals[J]. , 2012,(2): 144-152
王英涛, 周璇, 张志东. 双轴向列相Fréedericksz转变的Monte Carlo模拟[J]. 液晶与显示, 2012,(2): 144-152 DOI：

WANG Ying-tao, ZHOU Xuan, ZHANG Zhi-dong. Monte Carlo Simulation of Fréedericksz Transitions in Biaxial Nematic Liquid Crystals[J]. , 2012,(2): 144-152 DOI：

摘要

粘连细胞的分割是医学细胞图像处理中的关键，随着形态学在图像分割中的应用，分水岭分割成为粘连细胞分割中最有效的方法之一。然而，由于巨噬细胞图像中的噪声和形态的不规则性，传统分水岭算法容易产生过分割。若在经距离变换得到的距离图中提取“种子点”，并根据距离图信息得到“种子点”合并的阈值，若两“种子点”之间的距离小于阈值，则将这两“种子点”合并。若在分水岭分割中使用根据新“种子点”重新分布的距离图，实验结果表明：与传统方法相比，该算法虽然要多花费些时间，但可以有效地分割粘连巨噬细胞并抑制过分割现象。分割后，对细胞进行标记并提取细胞的特征参数，如周长、面积、圆度因子、灰度均值等。

Abstract

Segmentation to adhesion cells is critical in image segmentation realm

with the morphology was used in image segmentation

watershed becomes one of the most efficient skills in solving adhesion cells. However

the traditional watershed algorithm is apt to cause over-segmentation for the noise and irregular shape of macrophages.If extracting the "seeding points" from the distance map

and combining some "seeding points" into more reasonable "seeding points" by a certain criterion that the neighbor "seeding points" should be combined to one "seeding point" if the distance between the two points is under threshold which is got from the information the distance map indicates.And redistribute distance map depending on the "seeding points" before watershed

the simulation results show that the algorithm can effectively segment adhesion macrophages as well as restrain the over-segmentation phenomena with a little more time compared with the traditional method.After segmentation

we can mark the numbers to the cells and abstract the characteristic parameters such as circumference、area、roundness factor 、mean gray and so on.

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Keywords

references

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