Influence of Cholesterol on Structure of Phosphatidylcholine／Ceramide of Duality System

LIU Xiao-yan; HAO Chang-chun; SUN Run-guang

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Influence of Cholesterol on Structure of Phosphatidylcholine／Ceramide of Duality System

Material Physics & Chemistry | 更新时间：2020-08-12

- Influence of Cholesterol on Structure of Phosphatidylcholine／Ceramide of Duality System
- Chinese Journal of Liquid Crystals and Displays Vol. 24, Issue 6, Pages: 794-798(2009)
- 作者机构：
  
  1. 西安文理学院物理系,陕西西安 710065, E-mail:lxy881188@126.com
  2. 陕西师范大学物理学与信息技术学院生物物理与生物医学工程研究室,陕西西安 710062
- 作者简介：
- 基金信息：
- DOI：
  CLC： O753+.2
- Received：13 May 2009，
  
  Revised：02 January 1900，
  
  Published Online：30 December 2009，
  
  Published：30 December 2009
- 稿件说明：
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刘晓燕;郝长春;孙润广. 胆固醇对磷脂酰胆碱/神经酰胺二元体系结构的影响[J]. 液晶与显示, 2009,24(6): 794-798

LIU Xiao-yan;HAO Chang-chun;SUN Run-guang. Influence of Cholesterol on Structure of Phosphatidylcholine／Ceramide of Duality System[J]. 液晶与显示, 2009,24(6): 794-798
刘晓燕;郝长春;孙润广. 胆固醇对磷脂酰胆碱/神经酰胺二元体系结构的影响[J]. 液晶与显示, 2009,24(6): 794-798 DOI：

LIU Xiao-yan;HAO Chang-chun;SUN Run-guang. Influence of Cholesterol on Structure of Phosphatidylcholine／Ceramide of Duality System[J]. 液晶与显示, 2009,24(6): 794-798 DOI：

摘要

通过LB膜仪对表面压力-面积(

-A

)曲线测量及原子力显微镜(AFM)观测

研究了胆固醇对磷脂酰胆碱/神经酰胺二元体系结构的影响。-A曲线分析表明

随着胆固醇的增加

加速了相变的发生

促进了膜稳定状态的形成;AFM观察结果表明

磷脂酰胆碱/神经酰胺(PC/CER)二元混合系统单分子膜呈现为小的微区结构

当PC/CER二元混合系统与胆固醇混合时

随着胆固醇比例的增加

混合膜呈现从链状结构向较大的片状与网状共存结构的转化

最终纯胆固醇形成高度紧密的膜结构。

Abstract

This paper investigated the effect of cholesterol on the structures of the phosphatidylcholine (PC) and ceramide(CER) of duality system by the pressure-area(

-A

) isotherm measurements and by atomforce microscopy(AFM) observation. It is shown from the

-A

isotherms that the increase of cholesterol can accelerate the occurrence of phase change and advance the stabilization of films. Also

it is found from the observation of AFM that the duality systems (PC/CER)molecular film takes on microregion structure. With the increasing of cholesterol content

the mixed films transforms from the chains structure to larger slice and net coexisted structure. In the end

pure cholesterol forms more aggregated structure.

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Keywords

references

. Boxer S G. Molecular transport and organization in supported lipid membranes [J]. Curr. OPin Chem. Biol., 2000,4(6):704-709.

. Sun Run Guang,Zhang Jing,Wang Yong Chang. Conformation of the liquid crystal state in the interaction between several chemical material and biomembrane [J]. Chin. Science, 1998,4(1):1-13.

. 孙润广,张静,王永昌.脂肪酸多相脂质体与癌细胞膜相互租用的ESR谱技术研究 [J]. 生物化学与生物物理进展, 2000,27(5):296-300.

. 孙润广,张静,齐浩,等.胆固醇对双层结构影响的SAXS和STM研究 [J]. 生物物理学 ,2001,17(1):53-58.

. 杨福愉.生物膜结构研究的一些进展 [J]. 生物化学与生物物理进展, 2003,30(4):495-502

. 欧阳钟灿.生物膜形状的液晶模型理论研究 [J]. 液晶与显示 ,2003,18(1):79-83.

. 何平笙.空气/水界面单分子膜的弹性 [J]. 化学通报, 2001,64(10):608-613.

. 孙润广,张静.原子力显微镜观察脂胞囊形貌结构及稳定性 [J]. 原子与分子物理学报, 2003,20(1):85-88.

. 杨福愉. 生物膜. [M]. 北京:科学出版社 , 2005:53.

. Scheiffele P, Simons K, Roth M G. Interaetion of influenza virus haemagglutinin with Sphingolipid- Cholesterol membrane domains viaits transmembrane domain [J].EMBOJ,1997,16(18):5501-5088.

. Simons K, Vaz W L.Model systems,lipid rafts,and cell membranes [J]. Annu Rev Bio Phys. Biomol. Struct.,2004,33(1):269-295.

. Ipsen J H, Karlstim G, Mouritsen O G, et al. Phase equilibria in the Phosphatidylcholine-cholesterol system [J]. Biochim.BioPhys. Aeta, 1987,905(1):162-172.

. 杨小乐,孙润广,张静.LB膜与AFM技术研究磷脂酰乙醇胺单分子膜结构 [J]. 液晶与显示 ,2006,21(4):348-355.

. 杨福愉. 生物膜 [M].北京:科学出版社,2005:386.

. Dawn Y Takamoto,Eray Aydil,JosePh A, et al.Stable ordering in Langmuir-Blodgett films [J] .Science, 2001,293:1292-1297.

. 郝长春,孙润广. Langmuir-Blodgett膜超分子SM／DOPC／Chol三元脂系的原子力显微镜观察 [J]. 化学学报, 2007,65(20):2325-2330.

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